The University of Southampton

Dr Jonathan Butement PhD, BSc

Research Fellow ECS

Jonathan is a research fellow in the School of Electronics and Computer Science, with cross-disciplinary expertise (BSc Biomedical Science, PhD Optoelectronics). His work is focused on using integrated optical technology and microfluidics for biomedical applications most notably microflow cytometry.


- Deployable microflow cytometer for impedance and fluorescence based analysis of marine phytoplankton.

- Microflow cytometer for the analysis of pigment content of commercial algae crops.

- Microflow cytometer for the analysis of extracellular vesicle sized particles using multiangle scattering analysis

- "Algae Farmer" interactive game for science communication and outreach.

- Antimicrobial light in urinary catheters.

Jonathan has a broad skill base in optical theory and design, microfabrication, microfluidics, electronics and experimental biology. JB has been awarded £16K as PI from HEFCE (Zepler Institute Stimulus Fund) for research into anitmicrobial light guiding technology in biomedical devices. He has also been the researcher co-I on a £20K Higher Education Innovation Fund projects from HEFCE (Zepler Institute Stimulus fund). In 2019 JB was funded to attend the British Council/Newton fund, UK-India Researcher Links Multi-disciplinary Workshop on TB Diagnostics and AMR.


Research interests

Microflow cytometry, Integrated optics, Microfluidics, Lab-on-a-chip, Biosensing, Antimicrobial photonics


Jonathan has supervised masters students in interdisciplinary discertation projects.


Braddick, Lucy M., Garland, Patrick J., Praeger, Matthew F., Butement, Jonathan, Friedrich, Daniel, Morgan, David J. and Melvin, Tracy (2012) Uniform aligned bioconjugation of biomolecule motifs for integration within microfabricated microfluidic devices. Analytical Biochemistry, 424 (2), 195-205. (doi:10.1016/j.ab.2012.02.020).

Sones, C.L., Katis, I.N., Mills, B., Feinäugle, M., Mosayyebi, Ali, Butement, J. and Eason, R.W. (2014) Rapid and mask-less laser-processing technique for the fabrication of microstructures in polydimethylsiloxane. Applied Surface Science, 298, 125-129. (doi:10.1016/j.apsusc.2014.01.138).

Sones, C.L., Katis, I.N., He, P., Mills, B., Mosayyebi, A., Butement, Jonathan, Feinäugle, M. and Eason, R.W. (2014) Laser-based printing and patterning for biological applications. International Workshop on the Fabrication and Application of Microstructured Optical Devices, Yokohama, Japan. 27 - 28 Feb 2014.

Butement, J.T.G., Hunt, H.C., Rowe, D.J., Karabchevsky, Alina, Hua, P., Murugan, G.S., Clark, O., Holmes, C., Carpenter, L.G., Gates, J.C., Smith, P.G.R, Chad, J.E. and Wilkinson, J.S. (2014) A microflow cytometer for microsphere-based immunoassays using integrated optics and inertial particle focussing. Biosensors '14, Melbourne, Australia. 26 - 29 May 2014.

Sones, C.L., Katis, I.N., Mills, B., Feinäugle, M., Mosayyebi, Ali, Butement, Jonathan and Eason, R.W. (2013) Rapid, low-cost patterning of microstructures in polydimethylsiloxane via mask-less laser-machining. 2013 Conference on Lasers & Electro-Optics Europe & International Quantum Electronics Conference CLEO EUROPE/IQEC, , Munich, Germany. 12 - 16 May 2013. (doi:10.1109/CLEOE-IQEC.2013.6801592).

Sones, C.L., Katis, I.N., Mills, B., Feinäugle, M., Mosayyebi, Ali, Butement, Jonathan and Eason, R.W. (2013) Mask-less laser-machining for rapid low-cost patterning of microstructures in polydimethylsiloxane (PDMS). E-MRS '13 Materials Research Society Spring Meeting, , Strasbourg, France. 27 - 31 May 2013.

Mittal, Vinita, Aghajani, Armen, Carpenter, Lewis, Gates, James C., Butement, Jonathan, Smith, Peter G.R., Wilkinson, James S. and Murugan, Ganapathy Senthil (2015) Fabrication and characterization of high-contrast mid-infrared GeTe4 channel waveguides. Optics Letters, 40 (9), 2016-2019. (doi:10.1364/OL.40.002016).

Butement, Jonathan, Hunt, Hamish, Rowe, David, Sessions, Neil, Clark, Owain, Hua, Ping, Murugan, G. Senthil, Chad, John and Wilkinson, James (2016) Integrated optical waveguides and inertial focussing microfluidics in silica for microflow cytometry applications. Journal of Micromechanics and Microengineering, 26 (10), [105004]. (doi:10.1088/0960-1317/26/10/105004).

Butement, Jonathan (2016) Integrated optical microflow cytometer for bead-based immunoassays. University of Southampton, Faculty of Physical Sciences and Engineering, Doctoral Thesis, 152pp.

Holloway, Paul M., Butement, Jonathan, Hegde, Manjunath and West, Jonathan (2018) Serial integration of Dean-structured sample cores with linear inertial focussing for enhanced particle and cell sorting. Biomicrofluidics, 12 (4), [044104]. (doi:10.1063/1.5038965).

Lane, Simon, Butement, Jonathan, Harrington, Jack, Underwood, Timothy, Shrimpton, John and West, Jonathan (2019) Perpetual sedimentation for the continuous delivery of particulate suspensions. Lab on a Chip, 19, 1-5. (doi:10.1039/C9LC00774A).

Butement, Jonathan (2020) Dataset for: Monolithically-integrated cytometer for measuring particle diameter in the extracellular vesicle size range using multi-angle scattering. University of Southampton doi:10.5258/SOTON/D1173 [Dataset]

Butement, Jonathan, Holloway, Paul, Welsh, Joshua A, Holloway, Judith, Englyst, Nicola, Horak, Peter, West, Jonathan and Wilkinson, James S (2020) Monolithically-integrated cytometer for measuring particle diameter in the extracellular vesicle size range using multi-angle scattering. Lab on a Chip, 20 (7), 1267-1280. (doi:10.1039/C9LC01182J).

Harrington, Jack, Esteban, Luis Blay, Butement, Jonathan, Vallejo, Andres F, Lane, Simon I R, Sheth, Bhavwanti, Jongen, Maaike S A, Parker, Rachel, Stumpf, Patrick S, Smith, Rosanna C G, MacArthur, Ben D, Rose-Zerilli, Matthew J J, Polak, Marta E, Underwood, Tim and West, Jonathan (2021) Dual dean entrainment with volume ratio modulation for efficient droplet co-encapsulation: extreme single-cell indexing. Lab on a Chip, 21 (17), 3378-3386. (doi:10.1039/d1lc00292a).

Qi, Yanli, Zheng, Zhexuan, Banakar, Mehdi, Wu, Yangbo, Gangnaik, Anushka, Rowe, David, Mittal, Vinita, Butement, Jonathan, Wilkinson, James S, Mashanovich, Goran and Nedeljković, Miloš (2021) Integrated switching circuit for low-noise self-referenced mid-infrared absorption sensing using silicon waveguides. IEEE Photonics Journal, 13 (6), [6600110]. (doi:10.1109/JPHOT.2021.3121331).


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